Technical Research Assistant II RESEARCH NEUROLOGY

Working very independently, under minimal supervision, executes protocols involving a variety of routine and non-routine experiments at a professional level. Develops modification of existing techniques, as appropriate. In collaboration with other members of the research team, gives input to research design. Calculates, transcribes, and analyzes data using statistical techniques. May be responsible for orienting and training new staff. 

May serve in a supervisory role in smaller lab.
PRINCIPAL DUTIES AND RESPONSIBILITIES:

• Under general supervision breeds and maintains common use mouse strains and use molecular techniques (PCR, quantitative PCR) and cellular techniques (flow cytometry) to genotype transgenic and knockout mice.
• Maintains basic records of mouse breeding, methodologies and test results in a log of work performed.
• Performs basic cell culture and in vitro alpha-synuclein assays by western blotting, and examination of receptor expression level by ELISA, intracytoplasmic staining, and cytometric bead array.
• Performs RNA quantification, tissue RNA isolation and RNA quality analysis by commercially available kits and protocols.
• Performs in vivo experiments involving behavioral studies such as rotarod and pole testing, open field testing and clasping scores and uses ImageJ-analyses or other software for measurement.
• Assists in the orientation and training of new staff in established laboratory techniques.
• Coordinates daily schedule of experiments.
• Responsible for data management: collects, records, tabulates and presents data. Creates graphs of experimental, performs statistical tests, and discusses progress of work regularly with supervisor.
• Uses judgment during experimental execution and meticulously performs procedures described laboratory protocols, research journals or devised with immediate supervisor.
• Basic understanding in light and confocal microscopy.
• All other duties as assigned.

QUALIFICATIONS:
Must have a Bachelor's degree and at least one-year experience in a directly related research setting.

SKILLS/ABILITIES/COMPETENCIES REQUIRED:

• High degree of computer literacy.
• Sound analytical and organizational skills.
• Good oral and written communication skills.
• Structures logically and effectively tasks and set priorities.
• Is able to identify potential problems and trouble-shoot solutions.
• Demonstrates ability to analyze data statistically and to logically present it.
• Shows strong interpersonal communication skills.

WORKING CONDITIONS:

• Normal laboratory environment.
• Hazardous materials are used in some of the experiments conducted.
• Animal tissue will be used.

SUPERVISORY RESPONSIBILITY:
Responsible for training and orienting new staff. May serve as a team leader or supervisor in a smaller research setting.

BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.