New

Research Laboratory Technician I

Massachusetts General Hospital
United States, Massachusetts, Boston
60 Blossom Street (Show on map)
Mar 05, 2025
A Research Laboratory Technician position is available in the laboratory of Dr. Marc Wein in the Endocrine Unit at the Massachusetts General Hospital. The Research Laboratory Technician I is a full-time position, and the primary responsibility is to assist in ongoing research studies of bone under the supervision of a Principal Investigator. The MGH Endocrine Unit is a leader in investigating, diagnosing and treating disorders of bone and mineral metabolism, thyroid diseases and general endocrine conditions. Responsibilities include collection of and accurate recording of procedures and results, working with mice, maintenance of databases used in the lab, ordering necessary reagents to conduct experiments, and participation in joint upkeep of the lab. This position requires a person who has Bachelor of Science (or equivalent) degree in molecular/cell biology, biochemistry or a related field, pays attention to details, is capable of working independently under close supervision after initial training, possesses good verbal and written communication skills, and works well as part of a team. Work is conducted under direct supervision of a postdoctoral fellow and reviewed regularly with the Principal Investigator who is available to troubleshoot problems. Education BS/BA in biology, molecular biology, or biochemistry or related field is required. Experience * Prior laboratory experience, either through academic coursework, internships, or employment. 1-2 years preferred. * Experience with basic laboratory techniques in the setting of a research experience or undergraduate biochemistry/molecular biology lab eg: sterile cell culture techniques, microscopy, basic molecular biology techniques-such as plasmid purification, gel electrophoresis, PCR, immunoblotting, immunofluorescence, and mouse handling. * Ability to build upon these techniques, learning new ones as necessary, to conduct the research project. * Must be self-motivated and able to perform multiple tasks independently. * Excellent written and verbal communication skills. * Basic proficiency in Word, Power point, Excel, and Internet searches is required. Responsibilities comprise, but are not limited to: * Techniques used will include: mammalian cell culture, Western blotting, RNA analyses, PCR, plasmid cloning, CRISPR/Cas9 genome editing, plasmid preparations, animal handling (mice) and biologic sample collection, dissection of mice for histological analysis, immunohistochemistry. * Responsible for ordering common laboratory reagents and other supplies and for assuring that supply levels in the lab are adequate. Prepares materials for shipping, assuring compliance with regulations for biological samples. * Operates moderately complex equipment including microscopes, centrifuges, autoclave, spectrophotometers, PCR machines, plate readers, quantitative real-time PCR machines * Organizes and accurately maintains written records of experimental procedures and data collection. Data may be represented as graphs, charts, tables, illustrations, slides and narrative reports. Is able to learn to interpret data results and has a high degree of computer literacy. * Aids in maintaining information in databases for plasmids, cells, antibodies, and primers and boxes in the refrigerator, -20 and -80 freezers, and liquid nitrogen storage. * Performs basic design and modification of protocols. Executes protocols of non-routine experiments. * Participates in upkeep of lab, performs basic maintenance on laboratory equipment (cleans, disassembles, stores, replaces worn parts) and reports equipment requiring advanced repair. * Participates in and presents data at weekly lab meetings and journal clubs. * Interacts well with others, assists in training laboratory personnel in lab techniques and procedures that have been acquired. Working Conditions * Duties will be performed in a research laboratory. No direct patient contact will occur. * Must be willing to work with mice. * Work will be conducted over a 40-hour workweek 8:30AM-5:00PM Monday-Friday. The General Hospital Corporation is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.