Research Lab Tech I (Miyamoto's Lab)

The incumbent works as part of a multidisciplinary team and will be responsible for a range of activities including but not limited to:

• Isolation and analysis of circulating tumor cells (CTCs) from patient blood samples using microfluidic technologies.
• Molecular analysis of CTCs, tumors, and normal tissue samples using molecular biology and cellular biology techniques including RNA in situ hybridization, qPCR, droplet digital PCR, immunofluorescence, flow cytometry, and next-generation sequencing.
• Perform standard molecular biology techniques including nucleic acid purification, PCR, cloning, site-directed mutagenesis, and plasmid construction.
• Perform standard tissue culture and cell biology techniques including cell line maintenance, drug treatments, plasmid transfection, Western blotting, cell viability assays, and immunofluorescence microscopy.
• Perform bioinformatic analyses including differential gene expression profiling and genomic mutational landscape analysis of tumors.
• Order common laboratory reagents and general laboratory supplies.
• Participate in upkeep of the lab and basic maintenance of laboratory equipment.
• Organize and accurately maintain written records of experimental procedures and data collection.
• Participate in weekly lab meetings.
• Assist in preparation of manuscripts resulting from data generated in research projects.

• Able to work independently and as part of a team
• Excellent verbal and written communication skills
• Laboratory research techniques (training will be provided)
• Attention to details in experimental techniques and record keeping
• Facility with computer software for data analysis
• Excellent organizational skills, working well under pressure
• Multi-tasking