New 
Scientist
 Spectraforce Technologies | |
 United States, Massachusetts, Cambridge | |
 Mar 10, 2025 | |
|
Description:
Scientist 06 months (potential extension) Cambridge, Massachusetts, 2139 Lab-based position, will help process PBMCs and FFPE tissues for scRNAseq and fixed RNA profiling workflows, scRNAseq library preparation and QC What are the top 3-5 skills, experience or education required for this position? 1. Experience with scRNAseq workflows (10x Genomics preferred) including library preparation and QC is required 2. Experience handling PBMCs, primary cells, and/or tissues is required 3. Background in biology, immunology, molecular biology, or other related field 4. Experience with automated liquid handlers is preferred but not required 5. Experience working with large sample cohorts/in a team environment is preferred Key Responsibilities: * Execute high-throughput scRNAseq library preparations by working effectively on a team and leveraging the use of automated liquid handlers (e.g. Biomek i7). * Operate Illumina sequencers and other lab instrumentation for quality control of NGS Libraries (e.g., Tapestation). * Accurately document project and sample information, protocols, and experimental details through the Electronic Lab Notebook (ELN) * Conduct experiments, troubleshoot, and optimize protocols independently and in collaboration with colleagues to deliver high-quality data in a timely fashion. * Interpret findings and communicate effectively to present scientific data, concepts, and recommendations to various internal audiences. * Interface with computational biologists and data scientists to generate and interpret scRNAseq datasets. * Work in ""matrixed environment and support activities for different groups across the organization, which may include alignment meetings. Qualifications: * BS or equivalent education with 3+ years of experience or MS or equivalent education with typically 2+ years of experience. * Strong background in basic molecular biology and/or genomics with proficiency in molecular biology lab techniques such as DNA/RNA extraction and nucleic acid QC, as well as familiarity with PBMC isolation and handling Preferred Qualifications: * Experience with nuclei isolation from PBMCs and/or tissues is preferred. * Familiarity with 10X Genomics platforms, particularly scRNAseq, Fixed RNA profiling, and/or scATACseq, is preferred. * Familiarity with automated liquid handlers such as Biomek i7 or Tecan Fluent is a plus but not required. * Background in R is a plus but not required. * Background in immunology is a plus bu | |