RESEARCH ASSISTANT II- Ward Lab

ABOUT US: Scripps Research is ranked the most influential institution in the world for its impact on innovation. We expand basic knowledge in the biosciences and use these fundamental advancements to develop profound innovations that improve wellbeing. Our educational and training programs mold talented and committed students and postdocs into leading edge scientists. Scientists in the institute's five academic research departments work hand-in-hand with researchers of the Scripps Research Translational Institute and Calibr to merge foundational studies in biology, chemistry and computer science with translational science to produce pioneering drugs and advances in digital and precision medicine. Together, we cultivate new scientific leaders and expand the frontiers of knowledge to deliver medical breakthroughs to better human health around the globe. If you have a passion for making a difference, this could be your opportunity to join our transformative team.

POSITION TITLE: Research Assistant II

POSITION SUMMARY:

The Ward lab is dedicated to translating cutting-edge academic discoveries into preclinical biologics and providing expert cryoEM services. Through strategic partnerships with biotechnology companies and the development of internal R&D programs, the Ward lab bridges the gap between basic research and therapeutic innovation, driving forward novel biologics platforms and candidate development.

We are seeking a highly motivated Research Assistant II to support ongoing discovery efforts within our membrane protein biologics pipeline and structural characterization initiatives for external partnerships. The successful candidate will contribute to assay development, protein engineering, and structural biology workflows in a fast-paced, collaborative environment that combines academic innovation with a start-up culture.

RESPOSIBILITIES AND DUTIES:

• Work under minimal supervision. Independently conducts a wide variety of standard laboratory techniques/procedures while using judgement when necessary to adapt standard methods and techniques. Receive instructions on specific assignment objectives, complex features, and possible solutions.
• Provide interpretation and analyses of results. Troubleshoot and modify procedures.
• May assist in designing experiments to complete a segment of overall research plan. May provide routine equipment maintenance. May provide guidance/training to department.
• Cell Line Engineering: Generate stable reporter cell lines using lentiviral transduction methods.
• Assay Development: Design, implement, and optimize fluorescence- and flow cytometry-based assays for lead molecule discovery and validation.
• Protein Characterization: Perform biochemical analyses of proteins and protein complexes to support biologics discovery and structure-guided engineering efforts.
• Cross-Functional Support: Contribute to ongoing efforts in protein design, cell line engineering, functional assay development, and library screening as needed.
• Electron Microscopy (EM) and Electron Microscopy Polyclonal Epitope Mapping (EMPEM) Support: Prepare samples for negative-stain and cryo-electron microscopy; perform grid preparation, data collection, and data processing as needed. Support EMPEM workflows including protein purification, complex formation, and sample quality assessment to enable low and high-resolution epitope mapping of antigen-antibody interactions.
• Record Keeping: Maintain detailed experimental records, troubleshoot protocols, and effectively communicate findings with the team.

REQUIREMENTS:

• Requires a Bachelor's degree Biochemistry, Biomedical Engineering, or a related field.
• Requires 3-5 years' research experience.
• Independent, proactive problem-solver who thrives in a dynamic, fast-paced environment.
• Reliable, communicative, and team-oriented contributor with strong attention to detail.
• Proven ability to manage multiple projects in parallel.
• Hands-on experience with molecular cloning and immunostaining.
• Proficient in mammalian cell culture and transfection techniques.
• Experience with protein expression and purification workflows.
• Skilled in flow cytometry assay setup and data analysis.

ADDITIONAL EXPERIENCE

• Lentiviral vector production and stable cell line generation.
• Protein expression and purification (advanced or large-scale applications).
• Familiarity with structural biology methods and transmission electron microscopes.
• Exposure to computational or rational protein design approaches.

PHYSICAL REQUIREMENTS:

• This position works in a laboratory or vivarium environment.
• Details of established essential functions for this position will be addressed/discussed during the interview process.

COMPENSATION: The expected hiring range for this position is $68,480 to $77,000 per year, commensurate with experience. Consideration will be given to experience that exceeds the listed requirements.

COMPREHENSIVE BENEFITS INCLUDE:

• Eligibility for retirement plans with employer contribution.
• Full suite of Health and Welfare plans including three medical plan options (including an HSA available option), dental, vision, life insurance, disability, EAP and more.
• Access to Flexible Spending Accounts (Medical/Dependent Care).
• Remote/Hybrid/Flexible work schedules (Dependent on role).
• Competitive vacation and sick leave policies.
• Free, on-site Counseling Center which provides confidential counseling services to benefits-eligible employees and their family members.
• Free, on-site parking.

The above statements describe the level of work performed and expected in general terms. The statements are not intended to list all the responsibilities, duties and/or skills required of employees so classified, and the content herein is subject to change due to the business needs of Scripps Research, with or without notice. Furthermore, nothing in this job description shall be interpreted to be in conflict with or to eliminate or modify in any way the employment-at-will status of Scripps Research staff.

EEO Statement:

The Scripps Research Institute is an Equal Opportunity Employer. We promote diversity of thought, culture and background in the fields of science. All qualified applicants are encouraged to apply and will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other legally protected characteristic or status.