Specialist - Cancer Molecular Biology & Proteomics (Krogan lab, UCSF)

Specialist - Cancer Molecular Biology & Proteomics (Krogan lab, UCSF)

Your work will focus on mapping protein interactions and signaling networks for complex biological problems on cross-disciplinary projects. This role is an excellent opportunity to learn several valuable, cutting-edge techniques in cell and molecular biology, as well as network and systems biology with an emphasis in proteomics, while working with a dynamic and interdisciplinary research team. You will generate and characterize cell lines expressing affinity-tagged proteins of interest, perform affinity purifications, and proximity labeling of proteins and their interactors for mass spectrometry analysis. The candidate will be able to learn how to generate protein-interaction maps and other downstream analyses. The candidate will also have the opportunity to perform high-throughput functional genomics experiments, including CRISPR screens.

The Krogan Lab is a highly dynamic and collaborative laboratory focusing on the molecular networks underpinning infectious disease, cancer and neurological disorders.

Our ideal applicant is a problem solver with a positive attitude, excellent organizational and interpersonal skills, and the ability to learn and implement methodology efficiently. Candidate's CV and/or cover letter must state basic qualifications (or if pending) upon submission. Candidates must meet all qualifications by the time of hire.

Required Qualifications:

• Jr. Rank: Bachelor's degree in biological sciences or an appropriate scientific field.
• Assistant Rank: Master's degree, or Bachelor's degree and 3 years of work experience in biological sciences or an appropriate scientific field.
• Associate Rank: Master's degree, or Bachelor's degree and 5-10 years of work experience in biological sciences or an appropriate scientific field.
• Full Specialist: PhD. or 10 years of work experience in biological sciences or an appropriate scientific field.
• Experience in the essential molecular biology techniques of molecular cloning, PCR, and western blotting.
• Experience in cell/tissue culture.
• Able to work independently and meet deadlines; recognize and resolve problems while maintaining a high level of organization.
• Effective verbal and written communication skills to interface with the team to review and analyze the results.
• Demonstrated experience in Microsoft Word and Excel

Preferred Qualifications:

• Experience generating new cell lines and characterizing them.
• Experience culturing iPSCs and iPSC-derived cells.
• Experience transfection or viral transduction.
• Experience with affinity purifications of proteins.
• Experience with CRISPR/gene editing techniques
• Experience with immunoprecipitation.
• Experience in preparation of samples for mass spectrometric analysis.
• Experience in fluorescence microscopy.
• Experience with mass spectrometry data.
• Experience with managing large datasets and/or databases.
• Experience with statistical data analysis.

Please apply online at https://aprecruit.ucsf.edu/JPF05009, with a CV, cover letter, and three references.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $51,300-$181,800.

• 3 required (contact information only)

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.