Project Appointment Student

Position Description:

Position Summary

Project Appointment-STUDENT

Purpose

A Project Appointment-STUDENT (PA-STUDENT) classification is utilized for a foreign national who is at MCW as a student for the purposes of furthering his or her education. In order to qualify for this position, student status must be proven.

Attributes of a Project Appointment:

• PA-STUDENTS are temporarily brought into an MCW laboratory because they desire to supplement their education, which they are primarily receiving at an institution other than MCW.
• There is an established date of termination, and there is no expectation of continued or permanent employment.
• The total duration of the Project Appointment cannot exceed four years.

Primary Functions

Performs tissue culture procedures.

• Maintains cell lines by feeding, splitting, and passaging cells. Take care of cryopreservation and storage.
• Fractionates lymphocyte subsets using positive and negative selection and differential centrifugation techniques.
• Performs lymphocyte proliferation, cytotoxicity, apoptosis, and differentiation assays.
• Evaluate CAR-T-dependent cytotoxicity and programmed cell death.
• Performs in vivo mouse studies on malignant B and plasma cell lineage tumors.
• Monitors mice regularly for tumor onset and incidence. Genotype offspring rely mainly on PCR methods.
• Performs basic protein isolation and purification and SDS-PAGE-based analyses of purified and recombinant proteins, including Western blot analysis.
• Utilizes ELISA and other techniques for detection of lymphokines and other products.

Performs procedures in the field of recombinant DNA/RNA methodology.

• Performs PCR-based assays with appropriate techniques to avoid cross-contamination.
• Interprets DNA sequencing results.
• Assists in accurate record keeping and maintenance of laboratory databases, such as plasmids/vectors, probes, glycerol stocks, oligonucleotide primers, cDNA and PCR products, and fluorescent antibodies
• Assists in communicating experimental work and refining new techniques.
• Retrieves any background or supporting material from literature searches.
• Assists in preparing manuscripts/grants by organizing data and preparing photographs, computer-generated figures, and tables for publication or presentation as directed.
• Evaluate and record the experiment's outcome based on the goals and submit it for the investigator's inspection.
• Contributes to the smooth functioning of the laboratory.
• Maintains a clean work area.
• Interacts with personnel from other laboratories in a productive, professional manner.
• Accommodates new personnel and techniques.
• Completes work within time guidelines specified and discusses progress and any problems as needed.
• Assists in developing and monitoring quality control parameters, including institutional guidelines for handling /disposing hazardous materials.

Preferred Schedule:



Full-time role with expectations for coverage during core business hours and flexibility required as necessary to accommodate business needs.

Position Requirements:

Knowledge - Skills - Abilities

Required knowledge: biology, chemistry, mathematics, statistics, documentation, records management, data utilization, complex problem solving, critical thinking, resource management, and writing skills

Skills and abilities: This will vary based on the hiring manager's preferences and should include things that can be learned or trained for.

Specifications

Minimum Required Education: Must have student status at an accredited educational institution

Minimum Required Experience: None

Preferred Education: Student status

Preferred Experience: None

Field: Cancer biology, cancer immunology, molecular biology, immunotherapy

Certification: None

MCW as an Equal Opportunity Employer and Commitment to Non-Discrimination


The Medical College of Wisconsin (MCW) is an Equal Opportunity Employer. We are committed to fostering a diverse community of outstanding faculty, staff, and students, as well as ensuring equal educational opportunity, employment, and access to services, programs, and activities, without regard to an individual's race, color, national origin, religion, age, disability, sex, gender identity/expression, sexual orientation, marital status, pregnancy, predisposing genetic characteristic, or military status. Employees, students, applicants or other members of the MCW community (including but not limited to vendors, visitors, and guests) may not be subjected to harassment that is prohibited by law or treated adversely or retaliated against based upon a protected characteristic.

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