Technical Research Assistant II NEUROLOGY Kuchroo Lab

The position is for a TRA2 in the Kuchroo Lab at the Department of Neurology, BWH.

Working very independently, under very general supervision, executes protocols involving a variety of routine and non-routine experiments at a professional level. May identify new techniques. Develops modification of existing techniques, as appropriate. In collaboration with other members of the research team, gives input to the development of design protocols. Calculates, transcribes, and analyzes data using statistical techniques. May be responsible for orienting and training new staff.

This position involves extensive training at the initial stage, and hence requires a two-year commitment minimum.

PRINCIPAL DUTIES AND RESPONSIBILITIES:

• Independently performs routine and non-routine experiments, playing an integral role in wet laboratory techniques, including tissue harvesting, cell and microbial culture, and molecular biology methodologies.
• Animal husbandry experience, specifically breeding mouse strains, weanings, crosses, genotyping, cryopreservation, exporting mice and importing mice from outside collaborators.
• Experiments may include specific assays, tissue culture, PCR analyzing in vitro experiments and in vivo disease models (EAE, colitis, tumor models).
• Calculate, transcribes and analyzes data using advanced statistical techniques.
• Maintains long-term tissue cultures of human and mouse cell lines and monitors cells for viability and contamination.
• Performs literature searches and helps to prepare presentations and written articles for publication.
• In collaboration with PI or Research Manager, establishes new and modifies existing research techniques.
• Coordinates schedule of tests and procedures, contributing to the generation of protocols and documents.
• Coordinates lab activities such as infection control, radiation safety, and bio-safety activities.
• Prepares, orders and maintains stock of necessary lab reagents, solutions and supplies.
• Operates and maintains laboratory equipment.
• Assists in the orientation and training of new staff.
• All other duties as assigned.

QUALIFICATIONS:

• BS in a biological/physical science required.
• Must have at least one-year experience in a directly related research setting.
• Excellent interpersonal and collaborative skills.
• Ability to work independently and as part of a team.
• High level of initiative and ability to work with little supervision.
• Hands-on experience in molecular biology, genomic and cellular biology (cell-based assays, cell culture) helpful.
• Computer literacy and working knowledge of data management systems.

SKILLS/ABILITIES/COMPETENCIES REQUIRED:

• High degree of computer literacy.
• Sound analytical and organizational skills.
• Requires good oral and written communication skills.
• Must be able to logically and effectively structure tasks and set priorities.
• Ability to identify potential problems and trouble-shoot solutions.
• Demonstrated ability to analyze data statistically and to logically present it.

BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.